Modification of Antibodies

Fragmentation

Fab and F(ab)2 fragmentation including

  • Test cleavage of a small amount of antibody using Papain and Pepsin to determine the best cleavage conditions
  • Large-scale cleavage (up to 500 mg antibody) using the selected enzyme including quality control

Pair Search 

Antibodies will be biotinylated and used for the search of antibody pairs (capture and detector) by testing all antibodies against each other (checker-board titration). 

Conjugation

  • Conjugation with peroxidase and alkaline phosphatase
  • Labeling with fluorescent dyes
  • Biotinylation
  • Gold labelling: Production of colloid conjugates with defined particle size
  • Immobilization of antibodies on latex of magnetic beads with defined particle size
  • Coating of a carrier with antibodies (columns, plates, tubes etc.)

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