BioGenes' Antigen Requirements
Antigen Requirements for Monoclonal Antibody Production
We accept a variety of antigen types, including peptides, proteins, and haptens. To ensure the safety and quality of our processes, our in-house animal facilities and laboratories meet safety levels 1-2 (S1, S2) in accordance with the German Genetic Engineering Act (GenTG/GenTSV) and risk levels 1-3** as per the German Biological Substances Ordinance (BioStoffV).
Antigens are supplied in physiological buffer conditions, making them conducive for immunization. For optimal results, we recommend specific purity levels for antigens: >80% for immunization and >95% for screening. Falling short of the screening purity threshold may pose a risk of not achieving the desired product.
In terms of quantities, a typical project usually requires around 2 mg of antigen. However, we understand that different projects have varying needs, and we can adapt accordingly.
Please note that high levels of endotoxins are considered unsuitable for our development processes due to potential risks.
Antigen Requirements for Polyclonal Antibody Production
Various antigens are suitable for immunization in the polyclonal antibody development process. These options include cells, proteins, peptides, haptens, and even antibodies. Whether in solution, as cells, in gel form, or lyophilized, the different formats ensure that you can select the most appropriate delivery method for your immunization process.
Maintaining antigen purity is crucial for effective immunization. The recommended purity level for antigens is typically set at more than 85%, ensuring a high likelihood of achieving the desired antibody responses.
The quantity of antigen needed per animal can vary based on factors such as the host species and specific immunization protocol. Detailed protocols are available for protein and peptide immunization in rabbits, mice, rats, and sheep/goats to guide you in determining the appropriate quantities.
During the expert consultation phase, you'll have the opportunity to discuss essential information with our experts. Topics include selecting the host species, defining immunization protocols, and outlining antigen characteristics, such as size, tag, immunogenicity, and peptide sequence.
It's important to note that antigens with PEG-binding properties are not suitable for immunization, as they can interfere with antibody binding sites, potentially compromising the effectiveness of the immunization process.
Receipt of Goods/Antigens
BioGenes' incoming goods department is operating during week days (CET):
- Mon - Thr: 7am to 5pm
- Fri: 7am to 4pm
We can not guarantee proper processing for off-time shipments.
Please note that BioGenes will not be operating during the following bank holidays:
- 08 Mar 2024
- 29 March - 1 Apr 2024
- 01 May 2024
- 9 - 10 May 2024
- 20 May 2024
- 03 - 04 Oct 2024
- 23 Dec 2024 - 01 Jan 2025
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